Post-doc position, U. Washington

November 16, 2010 § Leave a comment

The Wolf-Yadlin lab is interested in understanding the mechanism of RTK-mediated signaling at a systems level. We focus in particular on TRKB – an RTK whose deregulation has been implicated in several types of cancer, most notably neuroblastoma, as well as in various diseases of the nervous system such as schizophrenia and Alzheimer’s disease. The approach we are taking involves the development and application of novel mass spectrometry- and microarray-based methodologies to quantitatively measure dynamic changes of large numbers of signaling proteins of interest. In combination with systematic perturbations of protein expression and function, these tools allow us to use a global approach to determine the connectivity of the RTK-mediated signaling networks and the dynamics of their signaling. In addition, we draw on well-established high-throughput gene expression profiling and phenotypic measurements to examine the relationships between signaling dynamics, gene expression, cellular phenotype and disease progression. We then use these quantitative data to develop computational models of cellular signaling and to explore correlations between protein activity and phenotype. Ultimately, we envision that these efforts will allow us to not only elucidate the topology of signaling networks, but to make informed predictions about the most beneficial intervention strategies to regulate a phenotype or ablate a disease.

Specific areas of research include:

– Developing new tools, in particular mass spectrometry based techniques to measure and quantify post-translational modification of proteins in complex biological contexts.

–     The study of complex biological systems and diseases such as cancer, diabetes, Alzheimer’s disease and other neurodegenerative diseases.

–     Developing computational models to understand the topology and dynamics of cellular signaling networks, their relationship with cellular phenotype and disease progression.

The Wolf-Yadlin lab is part of the Department of Genome Sciences at the University of Washington and it is also a member of the University of Washington Proteomics Resource. Postdoctoral positions are currently available. We are interested in candidates with strong backgrounds in molecular and cell biology, mass spectrometry and proteomics and/or quantitative sciences, who enjoy working in a collaborative environment and who are enthusiastic about developing and using high-throughput techniques and systems-level analyses to tackle questions in biological signal transduction.

To apply, email a cover letter describing your research interests, and a CV to ayadlin@uw.edu

Sample references:

Wolf-Yadlin A, Sevecka M, MacBeath G.  Dissecting protein function and signaling using protein microarrays. Curr Opin Chem Biol. 2009 Oct;13(4):398-405. Epub 2009 Aug 5. Review.

Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM. Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5860-5. Epub 2007 Mar 26.

Wolf-Yadlin A, Kumar N, Zhang Y, Hautaniemi S, Zaman M, Kim HD, Grantcharova V, Lauffenburger DA, White FM. Effects of HER2 overexpression on cell signaling networks governing proliferation and migration. Mol Syst Biol. 2006;2:54. Epub 2006 Oct 3.

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